Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the a? heterodimer.¹⁹

Additional reported applications (for the relevant formats) include: immunoprecipitation⁸, Western blotting⁸,?in vitro?blocking of mixed lymphocyte reactions^9,10, depeletion of MHC class II cells⁷,?and immunohistochemical staining of acetone-fixed frozen sections^4,5. For sensitive functional assays, we recommend using the Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) (Cat. No. 307648, 307665 - 307669).

1. Brodsky F. 1984.?Immunogenetics?19:179.
2. Robbins P,?et al.?1987.?Human Immunol.?18:301.
3. Stites D,?et al.?1986.?Clin. Immunol. Immunopathol.?38:161.
4. Warnke R,?et al.?1980.?J. Histochem. Cytochem.?28:771. (IHC)
5. Engleman E,?et al.?1981.?P. Natl. Acad. Sci. USA?78:1791. (IHC)
6. Zipf T,?et al.?1981.?Cancer Res.?41:4786.
7. Goodier M,?et al.?2000.?J. Immunol.?165:139. (Depletion)
8. Esser M,?et al.?2001.?J. Virol.?75:6173. (IP, WB)
9. Kalka-Moll WM,?et al.?2002.?J. Immunol.?169:6149. (Block)
10. Wang RF,?et al.?1999.?Science?284:1351. (Block)
11. Zaba LC,?et al.?2007.?J. Exp. Med.?204:3183.?PubMed
12. Fujita H,?et al.?2009.?P. Natl. Acad. Sci. USA?106:21795.?PubMed
13. Charles N,?et al.?2010.?Nat. Med.?16:701. (FC)?PubMed
14. Goncalves RM,?et al.?2010.?Infect. Immun.?78:4763.?PubMed
15. Yoshino N,?et al.?2000.?Exp. Anim. (Tokyo)?49:97. (FC)
16. Kim WK,?et al.?2006.?Am. J. Pathol.?168:822. (FC)
17. Stein R,?et al.?2011.?Leuk. Lymphoma?52:273.
18. Galkowska H,?et al.?1996.?Vet. Immunol. Immunopathol.?53:329.
19. Moro M,?et al.?2005.?BMC Immunol.?6:24.
20. Lauterbach N,?et al.?2014.?Mol Immunol.?59:19.?PubMed

GMP RUO Flow Cytometry Antibodies.?BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

• Batch-to-batch consistency
• Material traceability
• Documented procedures
• Documented employee training
• Equipment maintenance and monitoring records
• Lot-specific certificates of analysis
• Quality audits per ISO 13485:2016
• QA review of released products

B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs

1. Levacher M,?et al.?1990.?Clin. Exp. Immunol.?81:177.
2. Terstappen L,?et al.?1990.?J. Leukocyte Biol.?48:138.
3. Edwards JA,?et al.?1986.?J. Immunol.?137:490.
4. van Es A,?et al.?1984.?Transplantation?37:65.
5. O'Doherty U,?et al.?1994.?Immunology?82:487.
6. Thomas R,?et al.?1994.?J. Immunol.?153:4016.
7. Grouard G,?et al.?1996.?Nature?384:364.