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APC anti-human HLA-DR Antibody
产品名称：
APC anti-human HLA-DR Antibody
产品类别：
抗体
产品编号：
307609
产品应用：
307609
[价格]
规格 价格 库存
25tests ￥ 996 1

产品详情

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Chimpanzee, Dog, Common Marmoset, Squirrel Monkey, Cotton-topped Tamarin
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the a? heterodimer.19

Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7,?immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) (Cat. No. 307648, 307665 - 307669).

Application References

(PubMed link indicates BioLegend citation)
  1. Brodsky F. 1984. Immunogenetics 19:179.
  2. Robbins P, et al. 1987. Human Immunol. 18:301.
  3. Stites D, et al. 1986. Clin. Immunol. Immunopathol. 38:161.
  4. Warnke R, et al. 1980. J. Histochem. Cytochem. 28:771. (IHC)
  5. Engleman E, et al. 1981. P. Natl. Acad. Sci. USA 78:1791. (IHC)
  6. Zipf T, et al. 1981. Cancer Res. 41:4786.
  7. Goodier M, et al. 2000. J. Immunol. 165:139. (Depletion)
  8. Esser M, et al. 2001. J. Virol. 75:6173. (IP, WB)
  9. Kalka-Moll WM, et al. 2002. J. Immunol. 169:6149. (Block)
  10. Wang RF, et al. 1999. Science 284:1351. (Block)
  11. Zaba LC, et al. 2007. J. Exp. Med. 204:3183. PubMed
  12. Fujita H, et al. 2009. P. Natl. Acad. Sci. USA 106:21795. PubMed
  13. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  14. Goncalves RM, et al. 2010. Infect. Immun. 78:4763. PubMed
  15. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  16. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  17. Stein R, et al. 2011. Leuk. Lymphoma 52:273.
  18. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
  19. Moro M, et al. 2005. BMC Immunol. 6:24.
  20. Lauterbach N, et al. 2014. Mol Immunol. 59:19. PubMed
  21. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  22. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Zizzo G, et al. 2012. J Immunol. 189:3508. PubMed
  2. Vielle NJ, et al. 2018. Sci Rep. 8:5440. PubMed
  3. Wenthe J, et al. 2021. Cancer Immunology Immunotherapy. . PubMed
  4. Han J, et al. 2022. FEBS J. 289:417. PubMed
  5. Porter RL, et al. 2022. J Clin Invest. :. PubMed
  6. Parenti S, et al. 2021. NPJ Precis Oncol. 5:4. PubMed
  7. Alfaro C, et al. 2016. Clin Cancer Res. 22: 3924 - 3936. PubMed
  8. Ramaswamy A, et al. 2021. Immunity. 54(5):1083-1095.e7. PubMed
  9. M?czy?ska J, et al. 2020. Cell Death Dis. 1.073611111. PubMed
  10. Leite NC, et al. 2020. Cell Reports. 32(2):107894.. PubMed
  11. Wang Y, et al. 2019. J Transl Med. 17:93. PubMed
  12. Kotsiou E, et al. 2016. Blood. 128: 72 - 81. PubMed
  13. Liu Y, et al. 2022. iScience. 25:104405. PubMed
  14. Wu H, et al. 2022. Biomolecules. 12:. PubMed
  15. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  16. Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
  17. Jin Y et al. 2019. Nature communications. 10(1):391 . PubMed
  18. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  19. Li L, et al. 2015. J Am Soc Nephrol. 26: 2183-2197. PubMed
  20. Bsat M, et al. 2015. J Leukoc Biol. 98: 671 - 681. PubMed
  21. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  22. Cai J, et al. 2021. eLife. 10:00. PubMed
  23. Singh N, et al. 2017. J Endocrinol. 235:69. PubMed
  24. Yang M, et al. 2018. Oncol Lett. 15:3918. PubMed
  25. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  26. Nazari-Shafti TZ, et al. 2020. Biomolecules. 10:00. PubMed
  27. Scottà C, et al. 2016. Haematologica. 101: 91 - 100. PubMed
  28. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  29. Sasano T, et al. 2018. Clin Cancer Res. 24:4018. PubMed
  30. Seery V, et al. 2021. EBioMedicine. 67:103357. PubMed
  31. Cho JH, et al. 2021. Nat Commun. 12:792. PubMed
  32. Perera MR, et al. 2022. Int J Mol Sci. 23:. PubMed
  33. Li Z, et al. 2014. PLoS One. 9:106064. PubMed
  34. Xu L, et al. 2022. Int J Mol Sci. 23:. PubMed
  35. Roy Chowdhury R, et al. 2018. Nature. 560:644. PubMed
  36. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  37. Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed
  38. Johnson RK, et al. 2022. Sci Rep. 12:19920. PubMed
  39. Diamantopoulos PT, et al. 2022. Cancers (Basel). 14:. PubMed
  40. Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed
  41. Pate K, et al. 2015. J Infect Dis. 212: 1387 - 1396. PubMed
  42. Eitas T, et al. 2017. PLoS One. 10.1371/journal.pone.0184164. PubMed
  43. Garcia EG, et al. 2020. Leukemia. 35:679. PubMed
  44. Murphy DM, et al. 2022. J Clin Invest. Online ahead of print. PubMed
  45. Gorby C, et al. 2020. Sci Signal. :13. PubMed
RRID
AB_314687 (BioLegend Cat. No. 307609) AB_314688 (BioLegend Cat. No. 307610)

Antigen Details

Structure
Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD heavy and 27 kD light chain
Distribution

B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs

Function
Peptide presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leukocyte Biol. 48:138.
3. Edwards JA, et al. 1986. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364.

Gene ID
3122 View all products for this Gene ID 3123 View all products for this Gene ID
UniProt
View information about HLA-DR on UniProt.org

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