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Purified anti-human IL-32αβδ Anti
产品名称:
Purified anti-human IL-32αβδ Anti
产品类别:
抗体
产品编号:
513601
产品应用:
513601
[价格]
规格 价格 库存
50µg ¥ 1632 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IL-32α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
WB, ICC, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 4 - 6 ?g/mL?is recommended. To obtain a linear standard curve, serial dilutions of human IL-32 recombinant protein ranging from 2000 to 15 pg/mL?are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA1, 4 Capture: The purified KU32-56 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with biotinylated KU32-52 antibody as the detection antibody for measuring human IL-32.

Additional reported applications (for the relevant formats) include: Western blotting, immunofluorescence, and immunoprecipitation.

Clone KU32-56 is shown to be reactive towards IL-32 α, β, δ, but not the γ isoform and clone KU32-52 has been shown to bind to all isoforms of IL-32.1 Clone KU32-09 does not work for Western blotting.6

Application References

(PubMed link indicates BioLegend citation)
  1. Kim KH, et al. 2008. J. Immunol. Methods 333:38. (ELISA Capture)
  2. Kulkarni O,et al.2008. J Pharmacol Exp Ther. PubMed
  3. Greene CM, et al. 2010. AM J. Respir Crit Care Med. 181:31. PubMed
  4. Sakitani K, et al. 2012. Infect. Immun. 80:3795. (ELISA Capture) PubMed
  5. Li Y, et al. 2013. J Biol Chem. 288:20927. PubMed
  6. Seo Eun-hee, et al. 2008. J. Microbiol. Biotechnol. 9:1606-1612.
Product Citations
  1. Yun J, et al. 2018. Cell Death Dis. 3. PubMed
  2. Qu P, et al. 2021. J Autoimmun. 116:102559. PubMed
  3. CM G, et al. 2010. Am J Respir Crit Care Med. 2010181:31. PubMed
  4. Sakitani K, et al. 2012. Infect Immun. 80:3795. PubMed
  5. Li Y, et al. 2013. J Biol Chem. 288:20927. PubMed
  6. Kulkarni O, et al. 2009. J Pharmacol Exp Ther. 328:371. PubMed
RRID
AB_2248930 (BioLegend Cat. No. 513601)

Antigen Details

Structure
Cytokine
Distribution

Activated lymphocytes and NK cells

Cell Type
Lymphocytes, NK cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Kim KH, et al. 2008. J. Immunol. Methods 333:38.
2. Conti P, et al. 2007. Autoimmun. Rev. 6:131.
3. Chen Q, et al. 2006. Vitam Horm. 74:207.
4. Kim SH, et al. 2005. Immunity 22:131.
5. Cagnard N, et al. 2005. Eur. Cytokine Netw. 16:289.
6. Banda NK, et al. 2003. J. Immunol. 170:2100.

Gene ID
9235 View all products for this Gene ID
UniProt
View information about IL-32 on UniProt.org

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