Biotin anti-human IL-32αβγδ-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1908	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human IL-32α

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.2-1 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-32 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application

Application Notes

ELISA¹ Detection: The biotinylated KU32-52 antibody is useful as a detection antibody in a sandwich ELISA assay, when used in conjunction with purified KU32-07 or KU32-56 antibody as the capture antibody for measuring human IL-32.
Additional reported applications (for the relevant formats) include: Western blotting, immunofluorescence, and immunoprecipitation.

Clone KU32-52 has been shown to bind to all isoforms of IL-32 and clone KU32-56 is shown to be reactive towards IL-32 a, ?, d, but not the ? isoform.¹ Clone KU32-09 does not work for Western blotting.⁶

Application References (PubMed link indicates BioLegend citation)

Kim KH, et al. 2008. J. Immunol. Methods 333:38.
Greene CM, et al. 2009. Am J. Respir Crit Care Med. PubMed
Li W, et al. 2010. J. Immunol. 185:5056. PubMed
Soyka MB, et al. 2012. Allergy. DOI:10.1111/j.1398-9995.2012.02820.x. (FC, IF) PubMed
Hirata T, et al. 2012. Int Immunol. 24:705. PubMed.
Seo Eun-hee, et al. 2008. J. Microbiol. Biotechnol. 9:1606-1612.

Product Citations

Qu P, et al. 2021. J Autoimmun. 116:102559. PubMed
Gruber T, et al. 2020. JCI Insight. 5:00. PubMed

RRID

AB_2124017 (BioLegend Cat. No. 513503)

Antigen Details

Structure: Cytokine
Distribution: Activated lymphocytes and NK cells
Cell Type: T cells
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Kim KH, et al. 2008. J. Immunol. Methods 333:38.
2. Conti P, et al. 2007. Autoimmun. Rev. 6:131.
3. Chen Q, et al. 2006. Vitam Horm. 74:207.
4. Kim SH, et al. 2005. Immunity 22:131.
5. Cagnard N, et al. 2005. Eur. Cytokine Netw. 16:289.
6. Banda NK, et al. 2003. J. Immunol. 170:2100.
Gene ID: 9235 View all products for this Gene ID
UniProt: View information about IL-32 alpha beta gamma delta on UniProt.org

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