Alexa Fluor® 647 anti-mouse CD3ε Antib
- 产品名称：
- Alexa Fluor® 647 anti-mouse CD3ε Antib
- 产品类别：
- 抗体
- 产品编号：
- 155609
- 产品应用：
- 155609
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- CBAT6 Thymocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC -?Quality tested
IHC-F - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume.?For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- Tomonari K. 1988. Immunogenetics. 28:455. (FC)
- Product Citations
-
- Hildebrand KM, et al. 2021. PLoS One. 16:e0253864. PubMed
- RRID
- AB_2750435 (BioLegend Cat. No. 155609) AB_2750436 (BioLegend Cat. No. 155610)
- Structure
- Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD.
- Distribution
-
Thymocytes (differentiation dependent), mature T cells, NK-T cells
- Function
- TCR signal transduction, T cell activation
- Ligand/Receptor
- TCR
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
- Preston S, et al. 1997. Eur. J. Immunol. 27:1911.
- Davis MM. 1990. Annu. Rev. Biochem. 59:475.
- Weiss A, Littman DR. 1994. Cell. 76:263.
- Gene ID
- 12501 View all products for this Gene ID
- UniProt
- View information about CD3epsilon on UniProt.org
