Purified anti-human CD162 Antibody
- 产品名称：
- Purified anti-human CD162 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 328802
- 产品应用：
- 328802
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- PSGL-1 transfected murine 300.19 pre B-cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-P -?Verified
IHC-F, IP, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 ?g/mL.?It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone KPL-1 is reported to recognize the tyrosine sulfation consensus motif of PSGL-11.?Additional reported applications (for the relevant formats) include: Western Blot1, immunoprecipitation2, immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin embedded tissue sections1, blocks the recognition of PSGL-1 with P- and L-selectin1.
- Application References
(PubMed link indicates BioLegend citation) -
- Snapp KR, et al. 1998. Blood 91:154.
- Snapp KR, et al. 1998. J. Cell Biol. 142:263.
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Miyamura K, et al. 2011. J. Gen. Virol. 92:287. PubMed
- Cheng Q, et al. 2012. Lupus. 21:632. PubMed.
- Product Citations
-
- Weigert A, et al. 2022. Nat Commun. 13:6078. PubMed
- Cheng Q, et al. 2012. Lupus. 1.313888889. PubMed
- Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
- Perdomo J, et al. 2019. Nat Commun. 10:1322. PubMed
- Belmonte B, et al. 2021. Cancers (Basel). 13:. PubMed
- Miyamura K, et al. 2011. J Gen Virol. 92:287. PubMed
- Aleisa FA, et al. 2020. J Biol Chem. 295:3719. PubMed
- Donnelly C, et al. 2018. Sci Rep. 0.625. PubMed
- RRID
- AB_961085 (BioLegend Cat. No. 328802)
- Distribution
-
Neutrophils, monocytes, and most lymphocytes
- Function
- Mediates the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation.
- Ligand/Receptor
- CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin)
- Cell Type
- Lymphocytes, Monocytes, Neutrophils
- Biology Area
- Cell Adhesion, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Snapp KR, et al. 1998. Blood 91:154.
- Gene ID
- 6404 View all products for this Gene ID
- UniProt
- View information about CD162 on UniProt.org
