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PE anti-human CD162 Antibody
产品名称:
PE anti-human CD162 Antibody
产品类别:
抗体
产品编号:
328805
产品应用:
328805
[价格]
规格 价格 库存
25tests ¥ 1464 1

产品详情

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
PSGL-1 transfected murine 300.19 pre B-cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone KPL-1 is reported to recognize the tyrosine sulfation consensus motif of PSGL-11.?Additional reported applications (for the relevant formats) include: Western Blot1, immunoprecipitation2, immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin embedded tissue sections1, blocks the recognition of PSGL-1 with P- and L-selectin1.

Application References

(PubMed link indicates BioLegend citation)
  1. Snapp KR, et al. 1998. Blood 91:154.
  2. Snapp KR, et al. 1998. J. Cell Biol. 142:263.
  3. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  4. Miyamura K, et al. 2011. J. Gen. Virol. 92:287. PubMed
  5. Cheng Q, et al. 2012. Lupus. 21:632. PubMed.
Product Citations
  1. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
  2. Oggero S, et al. 2021. Journal of Extracellular Vesicles. 10(6):12084. PubMed
  3. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  4. Santos S, et al. 2011. Thromb Res. 127:105. PubMed
  5. Jamaly S, et al. 2018. J Thromb Haemost. 16(8):1546. PubMed
  6. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  7. Wang SS, et al. 2021. Cell Chemical Biology. 28(5):699-710.e5. PubMed
  8. Anderson NR, et al. 2019. Cell Adh Migr. 13:163. PubMed
RRID
AB_2185244 (BioLegend Cat. No. 328805) AB_2185243 (BioLegend Cat. No. 328806)

Antigen Details

Distribution

Neutrophils, monocytes, and most lymphocytes

Function
Mediates the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation.
Ligand/Receptor
CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin)
Cell Type
Lymphocytes, Monocytes, Neutrophils
Biology Area
Cell Adhesion, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Snapp KR, et al. 1998. Blood 91:154.

Gene ID
6404 View all products for this Gene ID
UniProt
View information about CD162 on UniProt.org

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