Brilliant Violet 421™ anti-human CD162 Antib
- 产品名称：
- Brilliant Violet 421™ anti-human CD162 Antib
- 产品类别：
- 抗体
- 产品编号：
- 328807
- 产品应用：
- 328807
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- PSGL-1 transfected murine 300.19 pre B-cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC?- Quality tested
IHC-P - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25??g per million cells in 100 ?L volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 ?g/mL?is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Clone KPL-1 is reported to recognize the tyrosine sulfation consensus motif of PSGL-11.?Additional reported applications (for the relevant formats) include: Western Blot1, immunoprecipitation2, immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin embedded tissue sections1, blocks the recognition of PSGL-1 with P- and L-selectin1.
- Application References
(PubMed link indicates BioLegend citation) -
- Snapp KR, et al. 1998. Blood 91:154.
- Snapp KR, et al. 1998. J. Cell Biol. 142:263.
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Miyamura K, et al. 2011. J. Gen. Virol. 92:287. PubMed
- Cheng Q, et al. 2012. Lupus. 21:632. PubMed.
- RRID
- AB_2749922 (BioLegend Cat. No. 328807) AB_2749923 (BioLegend Cat. No. 328808)
- Distribution
-
Neutrophils, monocytes, and most lymphocytes
- Function
- Mediates the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation.
- Ligand/Receptor
- CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin)
- Cell Type
- Lymphocytes, Monocytes, Neutrophils
- Biology Area
- Cell Adhesion, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Snapp KR, et al. 1998. Blood 91:154.
- Gene ID
- 6404 View all products for this Gene ID
- UniProt
- View information about CD162 on UniProt.org
