Alexa Fluor® 647 anti-human CD162 Antibody
- 产品名称：
- Alexa Fluor® 647 anti-human CD162 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 328809
- 产品应用：
- 328809
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1632 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- PSGL-1 transfected murine 300.19 pre B-cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-P - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.?For immunohistochemistry, a concentration range of 5.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Clone KPL-1 is reported to recognize the tyrosine sulfation consensus motif of PSGL-11.?Additional reported applications (for the relevant formats) include: Western Blot1, immunoprecipitation2, immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin embedded tissue sections1, blocks the recognition of PSGL-1 with P- and L-selectin1.
- Application References
(PubMed link indicates BioLegend citation) -
- Snapp KR, et al. 1998. Blood 91:154.
- Snapp KR, et al. 1998. J. Cell Biol. 142:263.
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Miyamura K, et al. 2011. J. Gen. Virol. 92:287. PubMed
- Cheng Q, et al. 2012. Lupus. 21:632. PubMed.
- Product Citations
-
- Albanese M, et al. 2022. Nat Methods. 19:81. PubMed
- RRID
- AB_2750409 (BioLegend Cat. No. 328809) AB_2750410 (BioLegend Cat. No. 328810)
- Distribution
-
Neutrophils, monocytes, and most lymphocytes
- Function
- Mediates the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation.
- Ligand/Receptor
- CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin)
- Cell Type
- Lymphocytes, Monocytes, Neutrophils
- Biology Area
- Cell Adhesion, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Snapp KR, et al. 1998. Blood 91:154.
- Gene ID
- 6404 View all products for this Gene ID
- UniProt
- View information about CD162 on UniProt.org
