Alexa Fluor® 647 anti-mouse TCR Vβ8.1, 8.
- 产品名称：
- Alexa Fluor® 647 anti-mouse TCR Vβ8.1, 8.
- 产品类别：
- 抗体
- 产品编号：
- 118414
- 产品应用：
- 118414
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- TCR receptor from T cell hybridoma DO-11.10
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- Reider SA, et al. 2011. Infect Immun. 79:3141. PubMed.
- Product Citations
-
- Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
- Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
- RRID
- AB_1186096 (BioLegend Cat. No. 118414)
- Structure
- TCR is a 85-kd hetero-dimer composed of alpha and beta chain. The mouse Vβ8 family has three members, namely Vβ8.1, 8.2 and 8.3.
- Distribution
-
Expressed on α:β T cells in mouse thymus, spleen and other lymphoid organs.
- Function
- Recognition of peptide/ MHC
- Interaction
- CD3, CD4, CD8
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Haskins K, et al. 1984. J. Exp. Med. 160:452
2. Behlke MA, et al. 1986. P. Natl. Acad. Sci. USA 83:767
3. Fairchild S, et al. 1992. 36:189
4. White J, et al. 1989. Cell 56:27
- Gene ID
- 21577 View all products for this Gene ID
- UniProt
- View information about TCR V beta 8.1 on UniProt.org
