Purified anti-human Ki-67 (Maxpar® Ready) Anti-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2052	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Cow

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Nuclei of the Hodgkin lymphoma cell line L428

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ICFC - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections¹, Western blotting³, and immunofluorescence microscopy⁴.

Ki-67 Staining Protocol:

1. Prepare 70% ethanol and chill at -20°C.
2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.
3. Discard supernatant and loosen the cell pellet by vortexing.
4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.
5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.
6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10⁶/ml.
7. Mix 100 ?l cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.
8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Gerdes J, et al. 1983. Int. J. Cancer 31:13. (IHC)
Gerdes J, et al. 1984. J. Immunol. 133:1710. (ICFC)
Schluter C, et al. 1993 J. Cell Biol. 123:513. (IHC, WB)
Bading H, et al. 1989 Exp. Cell. Res. 185:50. (IF)
Guha P, et al. 2013. PNAS. 110:5052. PubMed

Product Citations

Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
Senosain MF, et al. 2021. Sci Rep. 11:14424. PubMed
NULL, et al. 2022. Cell. 185:916. PubMed
Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed

RRID

AB_2562838 (BioLegend Cat. No. 350523)

Antigen Details

Structure: Two isoforms with molecular weights of 395 and 345 kD, one forkhead-associated domain, 16 concatenated Ki-67 repeats, located in nucleus
Distribution: Expressed in the phases G₁, S, G₂, and M of the cell cycle
Function: Required for cell proliferation
Interaction: Chromobox protein homolog 1, 3 and 5, Hklp2, and hNIFK
Biology Area: Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
Molecular Family: Nuclear Markers
Antigen References: 1. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
2. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
3. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
4. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
5. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID: 4288 View all products for this Gene ID
UniProt: View information about Ki-67 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们