PerCP/Cyanine5.5 anti-human Ki-67 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1632	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Cow

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Nuclei of the Hodgkin lymphoma cell line L428

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections¹, Western blotting³, and immunofluorescence microscopy⁴.

Ki-67 Staining Protocol:

1. Prepare 70% ethanol and chill at -20°C.
2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.
3. Discard supernatant and loosen the cell pellet by vortexing.
4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.
5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.
6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10⁶/ml.
7. Mix 100 ?l cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.
8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Gerdes J, et al. 1983. Int. J. Cancer 31:13. (IHC)
Gerdes J, et al. 1984. J. Immunol. 133:1710. (ICFC)
Schluter C, et al. 1993 J. Cell Biol. 123:513. (IHC, WB)
Bading H, et al. 1989 Exp. Cell. Res. 185:50. (IF)
Guha P, et al. 2013. PNAS. 110:5052. PubMed

Product Citations

Zhu S, et al. 2022. J Oncol. 2022:8724933. PubMed
Wang J, et al. 2022. Int J Mol Sci. 23:. PubMed
Wang YM, et al. 2022. Oncoimmunology. 11:2034257. PubMed
Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed

RRID

AB_2562294 (BioLegend Cat. No. 350519) AB_2562295 (BioLegend Cat. No. 350520)

Antigen Details

Structure: Two isoforms with molecular weights of 395 and 345 kD, one forkhead-associated domain, 16 concatenated Ki-67 repeats, located in nucleus
Distribution: Expressed in the phases G₁, S, G₂, and M of the cell cycle
Function: Required for cell proliferation
Interaction: Chromobox protein homolog 1, 3 and 5, Hklp2, and hNIFK
Biology Area: Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
Molecular Family: Nuclear Markers
Antigen References: 1. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
2. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
3. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
4. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
5. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID: 4288 View all products for this Gene ID
UniProt: View information about Ki-67 on UniProt.org

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