PE anti-human Ki-67 Antibody
- 产品名称：
- PE anti-human Ki-67 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 350503
- 产品应用：
- 350503
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1692 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Cow
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Nuclei of the Hodgkin lymphoma cell line L428
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.
Ki-67 Staining Protocol:
1. Prepare 70% ethanol and chill at -20°C.
2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.
3. Discard supernatant and loosen the cell pellet by vortexing.
4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.
5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.
6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.
7. Mix 100 ?l cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.
8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
- Application References
(PubMed link indicates BioLegend citation) -
- Gerdes J, et al. 1983. Int. J. Cancer 31:13. (IHC)
- Gerdes J, et al. 1984. J. Immunol. 133:1710. (ICFC)
- Schluter C, et al. 1993 J. Cell Biol. 123:513. (IHC, WB)
- Bading H, et al. 1989 Exp. Cell. Res. 185:50. (IF)
- Guha P, et al. 2013. PNAS. 110:5052. PubMed
- Product Citations
-
- Liu B, et al. 2015. J Virol. 89: 11834 - 11844. PubMed
- Taylor HE, et al. 2018. AIDS. 32:2847. PubMed
- Perreau M, et al. 2012. J Exp Med. 210:143. PubMed
- Hübner M, et al. 2020. Neurooncol Adv. 2:vdaa047. PubMed
- Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
- Muellenbeck M, et al. 2013. J Exp Med. 210:389. PubMed
- Sugita S, et al. 2016. Stem Cell Reports. 7:619-634. PubMed
- Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
- Wangler S, et al. 2019. Spine (Phila Pa 1976). 44:1613. PubMed
- Gao Y, et al. 2018. Oncol Lett. 15:4781. PubMed
- Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
- Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
- Jung M, et al. 2016. Sci Signal. 9: ra64. PubMed
- Fujii S, et al. 2019. Stem Cells Dev. 28:28. PubMed
- Su S et al. 2018. Cell. 175(2):442-457 . PubMed
- Timilshina M, et al. 2017. PLoS One. 10.1371/journal.pone.0168942. PubMed
- Pembroke T, et al. 2014. Gut. 63:515. PubMed
- Day C, et al. 2017. J Immunol. 10.4049/jimmunol.1700558. PubMed
- Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
- Makabe K, et al. 2019. Invest Ophthalmol Vis Sci. 60:1298. PubMed
- Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
- Su Y, et al. 2020. Cell. 1479:183. PubMed
- Hu X, et al. 2020. Neoplasia. 1.290972222. PubMed
- Seo IH, et al. 2021. Cell Reports. 36(4):109438. PubMed
- Li M, et al. 2020. Virol Sin. 35:588. PubMed
- RRID
- AB_10660818 (BioLegend Cat. No. 350503) AB_10660752 (BioLegend Cat. No. 350504)
- Structure
- Two isoforms with molecular weights of 395 and 345 kD, one forkhead-associated domain, 16 concatenated Ki-67 repeats, located in nucleus
- Distribution
-
Expressed in the phases G1, S, G2, and M of the cell cycle
- Function
- Required for cell proliferation
- Interaction
- Chromobox protein homolog 1, 3 and 5, Hklp2, and hNIFK
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
2. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
3. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
4. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
5. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
- Gene ID
- 4288 View all products for this Gene ID
- UniProt
- View information about Ki-67 on UniProt.org
