Alexa Fluor® 488 anti-human Ki-67 Antibody
- 产品名称：
- Alexa Fluor® 488 anti-human Ki-67 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 350507
- 产品应用：
- 350507
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1632 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Cow
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Nuclei of the Hodgkin lymphoma cell line L428
- Formulation
- ?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). - Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- ?g sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. For flow cytometric staining using test sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.
Ki-67 Staining Protocol:
1. Prepare 70% ethanol and chill at -20°C.
2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.
3. Discard supernatant and loosen the cell pellet by vortexing.
4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.
5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.
6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.
7. Mix 100 ?l cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.
8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
- Application References
(PubMed link indicates BioLegend citation) -
- Gerdes J, et al. 1983. Int. J. Cancer 31:13. (IHC)
- Gerdes J, et al. 1984. J. Immunol. 133:1710. (ICFC)
- Schluter C, et al. 1993 J. Cell Biol. 123:513. (IHC, WB)
- Bading H, et al. 1989 Exp. Cell. Res. 185:50. (IF)
- Guha P, et al. 2013. PNAS. 110:5052. PubMed
- Product Citations
-
- Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
- Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed
- Valpione S, et al. 2020. Nat Cancer. 0.1875. PubMed
- Remes Lenicov F, et al. 2018. Front Immunol. 9:1441. PubMed
- Maleki KT, et al. 2021. Cell Reports Medicine. 2(3):100220. PubMed
- Aerts-Kaya FSF, et al. 2020. Biol Blood Marrow Transplant. 634:26. PubMed
- Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed
- Luoma AM, et al. 2020. Cell. 182(3):655-671.e22. PubMed
- RRID
- AB_10900418 (BioLegend Cat. No. 350507) AB_2564169 (BioLegend Cat. No. 350532) AB_10933085 (BioLegend Cat. No. 350508)
- Structure
- Two isoforms with molecular weights of 395 and 345 kD, one forkhead-associated domain, 16 concatenated Ki-67 repeats, located in nucleus
- Distribution
-
Expressed in the phases G1, S, G2, and M of the cell cycle
- Function
- Required for cell proliferation
- Interaction
- Chromobox protein homolog 1, 3 and 5, Hklp2, and hNIFK
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
2. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
3. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
4. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
5. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
- Gene ID
- 4288 View all products for this Gene ID
- UniProt
- View information about Ki-67 on UniProt.org
