APC anti-mouse H-2D -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1224	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

C57BL/10 mouse skin graft and splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity¹, and Western blotting.

Application References (PubMed link indicates BioLegend citation)

Hasenkrug KJ, et al. 1987. Immunogenetics 25:136.
Shao H, et al. 2005. J. Immunol. 175:1851.
Ponomarev ED, et al. 2006. J. Immunol. 176:1402.
Robb RJ, et al. 2012 Blood. 119:5898. PubMed
Zhang P, et al. 2013. J. Immunol. 191:5291. PubMed
Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
Markey KA, et al. 2014. J Immunol. 192:5426. PubMed
Hogan T, et al. 2014. PLoS Comput Biol. 10:1003805. PubMed

Product Citations

Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
Wedekind MF, et al. 2021. iScience. 24(7):102759. PubMed
Boulch M, et al. 2021. Sci Immunol. 6:. PubMed

RRID

AB_2565861 (BioLegend Cat. No. 111513) AB_2565862 (BioLegend Cat. No. 111514)

Antigen Details

Structure: MHC class I
Distribution: Nucleated cells of the H-2D^b haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14964 View all products for this Gene ID
UniProt: View information about H-2Db on UniProt.org

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