Alexa Fluor® 647 anti-mouse H-2D
- 产品名称：
- Alexa Fluor® 647 anti-mouse H-2D
- 产品类别：
- 抗体
- 产品编号：
- 111512
- 产品应用：
- 111512
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- C57BL/10 mouse skin graft and splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, and Western blotting.
- Application References
(PubMed link indicates BioLegend citation) -
- Hasenkrug KJ, et al. 1987. Immunogenetics 25:136.
- Shao H, et al. 2005. J. Immunol. 175:1851.
- Ponomarev ED, et al. 2006. J. Immunol. 176:1402.
- Robb RJ, et al. 2012 Blood. 119:5898. PubMed
- Zhang P, et al. 2013. J. Immunol. 191:5291. PubMed
- Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
- Markey KA, et al. 2014. J Immunol. 192:5426. PubMed
- Hogan T, et al. 2014. PLoS Comput Biol. 10:1003805. PubMed
- Product Citations
-
- Milstein O, et al. 2011. Blood. 117:1042. PubMed
- Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
- RRID
- AB_492909 (BioLegend Cat. No. 111512)
- Structure
- MHC class I
- Distribution
-
Nucleated cells of the H-2Db haplotype
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD8
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
- Gene ID
- 14964 View all products for this Gene ID
- UniProt
- View information about H-2Db on UniProt.org
