Alexa Fluor® 647 anti-mouse I-A-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1212	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

C57BL/10 mouse splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity¹, and immunohistochemical staining² of acetone-fixed frozen tissue sections. Does not react with other haplotypes (e.g., a, d, k, n, p, q, u).

Application References (PubMed link indicates BioLegend citation)

Hasenkrug KJ, et al. 1987. Immunogenetics 25:136.
Pappo J, et al. 1999. Infect. Immun. 67:337.
Kamon H,et al.2006.EMBO J.25:4108.PubMed

Product Citations

Stoecklinger A, et al. 2011. J Immunol. 186:1377. PubMed

RRID

AB_493139 (BioLegend Cat. No. 115309) AB_492826 (BioLegend Cat. No. 115310)

Antigen Details

Structure: MHC class II
Distribution: B cell and activated T cells, APCs of H-2^b mice
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
Gene ID: 14961 View all products for this Gene ID
UniProt: View information about I-Ab on UniProt.org

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