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Purified anti-E2F1 Antibody
产品名称:
Purified anti-E2F1 Antibody
产品类别:
抗体
产品编号:
606051
产品应用:
606051
[价格]
规格 价格 库存
25µg ¥ 1416 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
GST fusion protein corresponding to full-length human E2F1. The epitope recognized by KH20 has been mapped to amino acids 1-89.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB- Quality tested
ICC, IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 ?g/mL.?For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 10.0 - 20.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product does not cross-react with mouse by WB.?

This antibody works by ICC using a variety of fixation permeabilization methods such as: fixation with either methanol or 4% PFA followed by permeabilization with either 0.5% Triton-X or 100% ice-cold methanol.

RRID
AB_2924616 (BioLegend Cat. No. 606051) AB_2924616 (BioLegend Cat. No. 606052)

Antigen Details

Structure
E2F1 is a 437 amino-acid protein with a predicted molecular weight of 46.9 kD
Distribution

Ubiquitously expressed/Nucleus

Function
Transcription factor/cell cycle
Interaction
E2F1 forms heterodimers with proteins of the Dimerization Partner (DP family).
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Cycle/DNA Replication, Transcription Factors
Antigen References
  1. Denechaud P, et al. 2017. Front. Endocrinol. 8:311.
  2. Liang Y, et al. 2015. Int. J. Oncol. 48:1650.
  3. Martinez-Balbas M, et al. 2000. Embo J. 19:662.
  4. Wu Z, et al. 2009. Int. J. Biochem. Cell Biol. 41:2389.
Gene ID
1869 View all products for this Gene ID
UniProt
View information about E2F1 on UniProt.org

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