Ultra-LEAF? Purified anti-mouse CD178.1 (FasL) Ant-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3240	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

C57BL/6 mouse FasL cDNA-transfected L5178Y cells

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking^1,2 and in vivo blocking^3-5, and immunohistochemical staining⁶ of formalin-fixed paraffin-embedded tissue sections. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106806) or by using a high sensitivity, three-layer staining technique (e.g., including biotinylated anti-mouse IgG (Cat. No. 405303) second step , followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 106809 and 106810).

Application References (PubMed link indicates BioLegend citation)

Kayagaki N, et al. 1997. P. Natl. Acad. Sci. USA 94:3914.
Kobata T, et al. 1997. Immunology 92:206.
Hattori K, et al. 1998. Blood 91:4051.
Takeda Y, et al. 1998. Diabetologia 41:315.
Kim YH, et al. 1999. Eur. J. Immunol. 29:455.
Takeda Y, et al. 1998. Diabetologia 41:315.

RRID

AB_2819812 (BioLegend Cat. No. 106809) AB_2819813 (BioLegend Cat. No. 106810)

Antigen Details

Structure: TNF superfamily Fas ligand alloantigen, 40 kD
Distribution: Activated T cells, spleen, testis, eye of some selected mouse strains (C57BL/6, C3H, NZB, NZw, MRL)
Function: Induces apoptosis
Ligand/Receptor: Fas (CD95)
Cell Type: T cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Nagata S. 1999. Annu. Rev. Genet. 33:209.
3. Takahashi T, et al. 1994. Cell 76:969.
4. Hill LL, et al. 1999. Science 285:898.
5. Kayagaki N, et al. 1997. P. Natl. Acad. Sci. USA 94:3914.
Gene ID: 14103 View all products for this Gene ID
UniProt: View information about CD178.1 on UniProt.org

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