PerCP/Cyanine5.5 anti-human Siglec-9 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2112	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant Siglec-9 fused to Fc region of human IgG

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence staining², Western blotting², immunoprecipitation², and ELISA³.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Zhang J, et al. 2000. J. Biol. Chem. 275:22121.
Avril T, et al. 2004. J. Immunol. 173:6841. (IF, IP, WB)
Biedermann B, et al. 2007. Leukemia Res. 31:221. (ELISA)

RRID

AB_2728332 (BioLegend Cat. No. 351517) AB_2728333 (BioLegend Cat. No. 351518)

Antigen Details

Structure: Immunoglobulin superfamily
Distribution: Highly expressed on neutrophils and monocytes, and at lower levels on the subpopulations of T cells and B lymphocytes and NK cells
Ligand/Receptor: Sialic acid
Cell Type: Monocytes, Neutrophils
Biology Area: Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: 1. Ikehara Y, et. al. 2004. J. Biol. Chem. 279:43117.
2. von Gunten S, et al. 2005. Blood 106:1423.
Gene ID: 27180 View all products for this Gene ID
UniProt: View information about Siglec-9 on UniProt.org

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