Purified anti-mouse CD1d Antibody -上海雅吉生物科技有限公司

规格	价格	库存
500µg	￥ 3036	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

α-GalCer:mCD1d complexes coupled to Mycobacterium tuberculosis PPD

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
Block, ELISA, ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA¹, immunofluorescence² to detect CD1d in the lipid rafts on the cell membrane, and block¹ CD1d recognition of an iNKT cell hybridoma.

Application References (PubMed link indicates BioLegend citation)

Yu KO, et al. 2007. J. Immunol. Methods 323:11. (ELISA, Block)
Im JS, et al. 2009. Immunity 30:888. (IF)

RRID

AB_10639731 (BioLegend Cat. No. 140802)

Antigen Details

Structure: Member of the MHC family. Type I transmembrane protein. Associates with β2-microglobulin. Molecular weight ranges from 43 - 49 kD, depending on glycosylation.
Distribution: Dendritic cells, monocytes, macrophages, B cells, thymocytes, intestinal epithelial cells
Function: Glycolipid presentation to iNKT cells
Ligand/Receptor: Vα14i TCR
Cell Type: B cells, Dendritic cells, Epithelial cells, Macrophages, Monocytes, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA 107:10984.
2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663.
3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA 107:1535.
4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241.
Gene ID: 12479 View all products for this Gene ID
UniProt: View information about CD1d on UniProt.org

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