PE anti-mouse CD1d Antibody
- 产品名称：
- PE anti-mouse CD1d Antibody
- 产品类别：
- 抗体
- 产品编号：
- 140805
- 产品应用：
- 140805
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- α-GalCer:mCD1d complexes coupled to Mycobacterium tuberculosis PPD
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume.? It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: ELISA1, immunofluorescence2 to detect CD1d in the lipid rafts on the cell membrane, and block1 CD1d recognition of an iNKT cell hybridoma.
- Application References
(PubMed link indicates BioLegend citation) -
- Yu KO, et al. 2007. J. Immunol. Methods 323:11. (ELISA, Block)
- Im JS, et al. 2009. Immunity 30:888. (IF)
- Product Citations
-
- Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
- Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
- RRID
- AB_10643277 (BioLegend Cat. No. 140805)
- Structure
- Member of the MHC family. Type I transmembrane protein. Associates with β2-microglobulin. Molecular weight ranges from 43 - 49 kD, depending on glycosylation.
- Distribution
- Dendritic cells, monocytes, macrophages, B cells, thymocytes, intestinal epithelial cells
- Function
- Glycolipid presentation to iNKT cells
- Ligand/Receptor
- Vα14i TCR
- Cell Type
- Dendritic cells, Monocytes, Macrophages, B cells, T cells, Epithelial cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA 107:10984.
2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663.
3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA 107:1535.
4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241.
- Gene ID
- 12479 View all products for this Gene ID
- UniProt
- View information about CD1d on UniProt.org
