APC anti-rat CD28 Antibody
- 产品名称：
- APC anti-rat CD28 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 200908
- 产品应用：
- 200908
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Rat CD28-transfected BALB/c mouse A2OJ cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
The JJ319 antibody has been reported to augment in vitro T cell proliferation and enhance IL-2 production. Additional reported applications (for the relevant formats) include: immunoprecipitation, in vitro costimulation of proliferation responses1, and in vitro costimulation of IL-2 production in the presence of R73 antibody (anti-rat a/? TCR, Cat. No. 201112)2.
- Application References
(PubMed link indicates BioLegend citation) -
- Tacke M, et al. 1995. J. Immunol. 154:5121. (Costim)
- Tacke M, et al. 1997. Eur. J. Immunol. 27:239. (Costim)
- Du Y, et al. 2012. PLoS One. 7:e52911. PubMed.
- RRID
- AB_2073971 (BioLegend Cat. No. 200908)
- Structure
- Ig superfamily, 44 kD
- Distribution
-
All α/βTCR+ T cells, most γ/δTCR+ T cells, NK cell subset
- Function
- T cell costimulation
- Ligand/Receptor
- CD80 (B7-1) and CD86 (B7-2)
- Cell Type
- T cells, NK cells
- Biology Area
- Immunology, Costimulatory Molecules
- Molecular Family
- CD Molecules
- Antigen References
-
1. Tacke M, et al. 1995. J. Immunol. 154:5121.
2. Mitnancht R, et al. 1995. Eur. J. Immunol. 25:328.
3. Tacke M, et al. 1997. Eur. J. Immunol. 27:239.
- Gene ID
- 25660 View all products for this Gene ID
- UniProt
- View information about CD28 on UniProt.org
