Spark Red™ 718 anti-mouse IL-2 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1284	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli-expressed, recombinant mouse IL-2

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark Red? 718 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.?

* Spark Red? 718 has a maximum excitation of 697 nm and a maximum emission of 718 nm.

Excitation Laser

Red Laser (633 nm)

Application Notes

ELISA Detection^1-3 or ELISPOT Detection^4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 & 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard.
Flow Cytometry^8-10: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations.
Neutralization^1,7: The Ultra-LEAF? purified antibody (Endotoxin in vivo and in vitro (Cat. No. 503845-503850)) is recommended for neutralization.
Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections², in vivo capture⁷, and immunocytochemistry.
Note: For testing mouse IL-2 in serum, plasma or supernatant, BioLegend's ELISA MAX? Sets (Cat. No. 431001 & 431004) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Abrams J, et al. 1992. Immunol. Rev. 127:5.
Sander B, et al. 1993. J. Immunol. Meth. 166:201.
Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20.
Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19.
Mo X, et al. 1995. J. Virol. 69:1288.
Karulin A, et al. 2000. J. Immunol. 164:1862.
Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
Ko SY, et al. 2005. J. Immunol. 175:3309. PubMed
Kang SS and Allen PM. 2005. J. Immunol. 174:5382.
Lawson BR, et al. 2007. J. Immunol. 178:5366.

Antigen Details

Structure: Cytokine; 15-30 kD (Mammalian)
Bioactivity: Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Cell Sources: T cells
Cell Targets: T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors: High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Cell Type: Tregs
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.
Regulation: Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)
Gene ID: 16183 View all products for this Gene ID
UniProt: View information about IL-2 on UniProt.org

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