Ultra-LEAF™ Purified anti-mouse IL-2 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1320	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli-expressed, recombinant mouse IL-2

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
ELISPOT Capture, Neut, IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISPOT capture applications, a concentration range of 2-6 ?g/ml is recommended. For ELISA capture applications, a concentration range of 0.5-2.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-2 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture^1-3 or ELISPOT Capture^4-6: The purified JES6-1A12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES6-5H4 antibody (Cat. No. 503804) as the detecting antibody and recombinant mouse IL-2 (Cat. No.575409) as the standard. The LEAF? purified antibody is suggested for ELISPOT capture (Cat. No. 503704).
Neutralization:?The JES6-1A12 antibody can neutralize the bioactivity of natural or recombinant IL-2.?For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 503706) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Abrams J, et al. 1992. Immunol. Rev. 127:5.
Sander B, et al. 1993. J. Immunol. Meth. 166:201.
Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19.
Mo X, et al. 1995. J. Virol. 69:1288.
Karulin A, et al. 2000. J. Immunol. 164:1862.
Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.

Product Citations

Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
Moore MJ et al. 2018. eLife. 7 pii: e33057. PubMed
Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed

RRID

AB_11150768 (BioLegend Cat. No. 503705) AB_11150775 (BioLegend Cat. No. 503706) AB_2814405 (BioLegend Cat. No. 503707) AB_2814406 (BioLegend Cat. No. 503708) AB_2814407 (BioLegend Cat. No. 503709) AB_2814408 (BioLegend Cat. No. 503710)

Antigen Details

Structure: Cytokine; 15-30 kD (Mammalian)
Bioactivity: Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Cell Sources: T cells
Cell Targets: T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors: High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Cell Type: Tregs
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.
Regulation: Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)
Gene ID: 16183 View all products for this Gene ID
UniProt: View information about IL-2 on UniProt.org

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