Purified anti-mouse IL-2 Antibody
- 产品名称：
- Purified anti-mouse IL-2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 503701
- 产品应用：
- 503701
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli-expressed, recombinant mouse IL-2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
IP, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-2 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Capture1-3 or ELISPOT Capture4-6: The purified JES6-1A12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES6-5H4 antibody (Cat. No. 503804) as the detecting antibody and recombinant mouse IL-2 (Cat. No.575409) as the standard. The LEAF? purified antibody is suggested for ELISPOT capture (Cat. No. 503704).
Neutralization:?The JES6-1A12 antibody can neutralize the bioactivity of natural or recombinant IL-2.?For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 503706) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).
- Application References
(PubMed link indicates BioLegend citation) -
- Abrams J, et al. 1992. Immunol. Rev. 127:5.
- Sander B, et al. 1993. J. Immunol. Meth. 166:201.
- Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
- Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19.
- Mo X, et al. 1995. J. Virol. 69:1288.
- Karulin A, et al. 2000. J. Immunol. 164:1862.
- Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.
- Product Citations
-
- Galaski J, et al. 2021. Front Cell Infect Microbiol. 11:692544. PubMed
- Nishio J, et al. 2010. J Exp Med. 207:1879. PubMed
- Lin YJ, et al. 2021. Cells. 10:. PubMed
- Secco V, et al. 2008. Biol Reprod. 78:234. PubMed
- Parrish H, et al. 2015. PLoS One. 10: 0132333. PubMed
- Newland SA, et al. 2017. Nat Commun. 8:15781. PubMed
- Zhao L, et al. 2021. STAR Protocols. 2(3):100732. PubMed
- Phan TS, et al. 2021. Sci Adv. 7:. PubMed
- Devine L, et al. 2006. J Immunol. 177:3930. PubMed
- RRID
- AB_315291 (BioLegend Cat. No. 503701) AB_315292 (BioLegend Cat. No. 503702)
- Structure
- Cytokine; 15-30 kD (Mammalian)
- Bioactivity
- Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
- Cell Sources
- T cells
- Cell Targets
- T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
- Receptors
- High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
- Cell Type
- Tregs
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.
- Regulation
- Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)
- Gene ID
- 16183 View all products for this Gene ID
- UniProt
- View information about IL-2 on UniProt.org
