热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
Ultra-LEAF™ Purified anti-mouse IL-10 Antibo
产品名称：
Ultra-LEAF™ Purified anti-mouse IL-10 Antibo
产品类别：
抗体
产品编号：
504907
产品应用：
504907
[价格]
规格 价格 库存
100µg ￥ 1320 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IL-10
Formulation
0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
Application

ELISA Capture - Quality tested
ELISPOT Capture, Neut, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA and ELISPOT capture applications, a concentration range of 4-8 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

* For ELISA/ELISPOT capture, it is very critical to use 0.2 M Sodium Phosphate Buffer, pH 6.5 (11.8g Na2HPO4, 16.1g NaH2PO4; q.s. to 1.0 L) as coating buffer.

Note: Carbonate buffer, pH 9.5 should not be used as coating buffer for JES5-2A5. It may cause high background and lower sensitivity.

Application Notes

ELISA or ELISPOT Capture1-5,7,10: The purified JES5-2A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES5-16E3 antibody (Cat. No. 505004) as the detecting antibody. The LEAF? purified antibody is suggested for ELISPOT capture.

ELISA or ELISPOT Detection: The biotinylated JES5-2A5 antibody is useful as the detecting antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Purified JES5-16E3 antibody (Cat. No. 505002/505012) as the capture antibody.

Neutralization2,8,9: The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse IL-10 bioactivity in vivo and in vitro (Cat. No. 504904).

Additional reported applications (for the relevant formats) include: Western blotting12 and in vivo capture6.

Note: For testing mouse IL-10 in serum, plasma or supernatant, BioLegend's ELISA Max? Sets (Cat. No. 431401 to 431406) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
  2. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  3. Abrams J, 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
  4. Mo X, et al. 1995. J. Virol. 69:1288.
  5. Sarawar S, et al. 1994. J. Immunol. 153:1246.
  6. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.
  7. Brummel R and Lenert P, 2005. J. Immunol. 174:2429.
  8. Hara M, et al. 2001. J. Immunol. 166:3789.
  9. Riemann M, et al. 2005. J. Immunol. 175:3560.
  10. Xu G, et al. 2007. J. Immunol. 179:5358.
  11. Guo Z, et al. 2013. J. Immunol. 190:4337. PubMed
  12. van der Poll T, et al. 1995. J. Immunol. 155:5397. (WB)
Product Citations
  1. Song J, et al. 2022. Clin Transl Med. 12:e992. PubMed
  2. Shute A, et al. 2021. Microbiome. 9:186. PubMed
RRID
AB_2810629 (BioLegend Cat. No. 504907) AB_2810630 (BioLegend Cat. No. 504908) AB_2810631 (BioLegend Cat. No. 504909) AB_2810632 (BioLegend Cat. No. 504910) AB_2810633 (BioLegend Cat. No. 504911) AB_2810634 (BioLegend Cat. No. 504912)

Antigen Details

Structure
Acid-labile cytokine; dimer; 17-21 kD (Mammalian)
Bioactivity
Inhibits IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppresses delayed type hypersensitivity response; stimulates TH2 cell response; mast cell proliferation
Cell Sources
Activated CD8+ T cells, TH0, TH2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
Cell Targets
T cells, B cells, mast cells, macrophages
Receptors
IL-10R (CDw210)
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. de Waal-Malefy, T, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard, M., et al. 1992. Immunol. Today. 13:198.
4. Quesniaux, V. 1992. Research Immunol. 143:385.

Regulation
Downregulated by IL-4, IL-10
Gene ID
16153 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线