Purified anti-mouse IL-10 (Maxpar® Ready) Anti-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1692	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli-expressed, recombinant mouse IL-10

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

ELISA or ELISPOT Detection^1,9,11: The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 & 504904) as the capture antibody.
ELISA Capture: The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. No. 505003) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard.
Neutralization¹⁴: The Ultra-LEAF? purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Flow Cytometry³: The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations.
Additional reported applications (for relevant formats) include: immunohistochemistry³.

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Simkin G, et al. 2000. J. Immunol. 164:2457.
Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
Khanna A, et al. 2000. J. Immunol. 164:1346.
Sander B, et al. 1993. J. Immunol. Methods 166:201.
Litton M, et al. 1994. J. Immunol. Methods 175:47.
Andersson U, et al. 1999. Detection and qunatification of gene expression. New York:Springer-Verlag.
Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
Wang W, et al. 2004. FASEB J. 18:1043.
Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
Lawson BR, et al. 2007. J. Immunol. 178:5366.
Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
Brummel R, et al. 2005. J. Immunol.174:2429. PubMed
Kang YJ, et al. 2007. Stem Cells 25:1814. PubMed
Seo N, et al. 2001. Immunology. 103:449. (Neut)

Product Citations

McDonald B, et al. 2020. Cell Host Microbe. 28(5):660-668.e4. PubMed

RRID

AB_2562846 (BioLegend Cat. No. 505029)

Antigen Details

Structure: Acid-labile cytokine, dimer, 17-21 kD (Mammalian)
Cell Sources: Activated CD8⁺ T cells, Th0, Th2 subset of CD4⁺ T cells, Ly-1⁺ B cells, monocytes, macrophages, keratinocytes
Cell Targets: T cells, B cells, mast cells, macrophages
Receptors: IL-10R (CDw210)
Cell Type: Tregs
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefy R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today 13:198.
4. Quesniaux V. 1992. Res. Immunol. 143:385.
5. Norton SK, et al. 2008. J. Immunol. 180:2848.
Regulation: Downregulated by IL-4, IL-10
Gene ID: 16153 View all products for this Gene ID
UniProt: View information about IL-10 on UniProt.org

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