FITC anti-mouse IL-10 Antibody
- 产品名称：
- FITC anti-mouse IL-10 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 505005
- 产品应用：
- 505005
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli-expressed, recombinant mouse IL-10
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
ELISA or ELISPOT Detection1,9,11: The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 & 504904) as the capture antibody.
ELISA Capture: The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. No. 505003) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard.
Neutralization14: The Ultra-LEAF? purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Flow Cytometry3: The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations.
Additional reported applications (for relevant formats) include: immunohistochemistry3.
- Application References
(PubMed link indicates BioLegend citation) -
- Simkin G, et al. 2000. J. Immunol. 164:2457.
- Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
- Khanna A, et al. 2000. J. Immunol. 164:1346.
- Sander B, et al. 1993. J. Immunol. Methods 166:201.
- Litton M, et al. 1994. J. Immunol. Methods 175:47.
- Andersson U, et al. 1999. Detection and qunatification of gene expression. New York:Springer-Verlag.
- Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
- Wang W, et al. 2004. FASEB J. 18:1043.
- Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
- Brummel R, et al. 2005. J. Immunol.174:2429. PubMed
- Kang YJ, et al. 2007. Stem Cells 25:1814. PubMed
- Seo N, et al. 2001. Immunology. 103:449. (Neut)
- Product Citations
-
- Prajeeth C,et al. 2017. J Neuroinflammation.. 10.1186/s12974-017-0978-3. PubMed
- Wang Y, et al. 2016. Sci Rep. 6:31881. PubMed
- Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
- Liu YJ, et al. 2020. Theranostics. 10:5225. PubMed
- Ma J, et al. 2021. Curr Protoc. 1:e144. PubMed
- Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed
- Dar HY, et al. 2018. Sci Rep. 8:2503. PubMed
- Li J, et al. 2018. Immunity. 49:178. PubMed
- Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
- Garg M, et al. 2021. Cell Reports. 34(6):108736. PubMed
- Reyes J, et al. 2016. Infect Immun . 84(12):3471-3483. PubMed
- Li Y, et al. 2021. Elife. 10:. PubMed
- Bedke T, et al. 2014. Immunol Cell Biol. . PubMed
- Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
- Jin R, et al. 2008. J Immunol. 180:2256. PubMed
- Sapra L, et al. 2021. Front Immunol. 12:691081. PubMed
- Sch?fer AL, et al. 2021. Front Immunol. 12:696810. PubMed
- Fearon AE, et al. 2021. iScience. 24:103143. PubMed
- Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
- Ge Y, et al. 2020. Mucosal Immunol. 13:34. PubMed
- RRID
- AB_315359 (BioLegend Cat. No. 505005) AB_315360 (BioLegend Cat. No. 505006)
- Structure
- Acid-labile cytokine, dimer, 17-21 kD (Mammalian)
- Cell Sources
- Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
- Cell Targets
- T cells, B cells, mast cells, macrophages
- Receptors
- IL-10R (CDw210)
- Cell Type
- Tregs
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefy R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today 13:198.
4. Quesniaux V. 1992. Res. Immunol. 143:385.
5. Norton SK, et al. 2008. J. Immunol. 180:2848.
- Regulation
- Downregulated by IL-4, IL-10
- Gene ID
- 16153 View all products for this Gene ID
- UniProt
- View information about IL-10 on UniProt.org
