Brilliant Violet 421™ anti-mouse IL-10 Antib
- 产品名称：
- Brilliant Violet 421™ anti-mouse IL-10 Antib
- 产品类别：
- 抗体
- 产品编号：
- 505021
- 产品应用：
- 505021
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli-expressed, recombinant mouse IL-10
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- ?g sizes: 0.2 mg/mL?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the ?g size, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. For immunofluorescent staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
ELISA or ELISPOT Detection1,9,11: The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 & 504904) as the capture antibody.
ELISA Capture: The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. No. 505003) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard.
Neutralization14: The Ultra-LEAF? purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Flow Cytometry3: The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations.
Additional reported applications (for relevant formats) include: immunohistochemistry3.
- Application References
(PubMed link indicates BioLegend citation) -
- Simkin G, et al. 2000. J. Immunol. 164:2457.
- Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
- Khanna A, et al. 2000. J. Immunol. 164:1346.
- Sander B, et al. 1993. J. Immunol. Methods 166:201.
- Litton M, et al. 1994. J. Immunol. Methods 175:47.
- Andersson U, et al. 1999. Detection and qunatification of gene expression. New York:Springer-Verlag.
- Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
- Wang W, et al. 2004. FASEB J. 18:1043.
- Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
- Brummel R, et al. 2005. J. Immunol.174:2429. PubMed
- Kang YJ, et al. 2007. Stem Cells 25:1814. PubMed
- Seo N, et al. 2001. Immunology. 103:449. (Neut)
- Product Citations
-
- Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
- Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
- Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
- Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed
- Hegde S, et al. 2020. Cancer Cell. 37(3):289-307. PubMed
- Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
- Lee CG, et al. 2019. JCI Insight. 4:5. PubMed
- Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
- Yasuma K, et al. 2016. PLoS Pathog. 12: 1005372. PubMed
- Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
- RRID
- AB_10900417 (BioLegend Cat. No. 505021) AB_2563240 (BioLegend Cat. No. 505022)
- Structure
- Acid-labile cytokine, dimer, 17-21 kD (Mammalian)
- Cell Sources
- Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
- Cell Targets
- T cells, B cells, mast cells, macrophages
- Receptors
- IL-10R (CDw210)
- Cell Type
- Tregs
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefy R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today 13:198.
4. Quesniaux V. 1992. Res. Immunol. 143:385.
5. Norton SK, et al. 2008. J. Immunol. 180:2848.
- Regulation
- Downregulated by IL-4, IL-10
- Gene ID
- 16153 View all products for this Gene ID
- UniProt
- View information about IL-10 on UniProt.org
