APC anti-mouse IL-10 Antibody
- 产品名称：
- APC anti-mouse IL-10 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 505009
- 产品应用：
- 505009
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli-expressed, recombinant mouse IL-10
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
ELISA or ELISPOT Detection1,9,11: The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 & 504904) as the capture antibody.
ELISA Capture: The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. No. 505003) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard.
Neutralization14: The Ultra-LEAF? purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Flow Cytometry3: The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations.
Additional reported applications (for relevant formats) include: immunohistochemistry3.
- Application References
(PubMed link indicates BioLegend citation) -
- Simkin G, et al. 2000. J. Immunol. 164:2457.
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- Litton M, et al. 1994. J. Immunol. Methods 175:47.
- Andersson U, et al. 1999. Detection and qunatification of gene expression. New York:Springer-Verlag.
- Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
- Wang W, et al. 2004. FASEB J. 18:1043.
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- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
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- Kang YJ, et al. 2007. Stem Cells 25:1814. PubMed
- Seo N, et al. 2001. Immunology. 103:449. (Neut)
- Product Citations
-
- Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
- Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
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- Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
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- Duan RN, et al. 2021. J Neuroinflammation. 18:145. PubMed
- Baier FA, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:745. PubMed
- Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
- Bochtler P, et al. 2008. J Immunol. 181:1633. PubMed
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- Novince CM, et al. 2017. Sci Rep. 7:5747. PubMed
- Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
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- Alhosaini K, et al. 2021. Brain Sci. :11. PubMed
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- B?hm L, et al. 2015. J Immunol. 194:887. PubMed
- Colliou N, et al. 2015. Toxins. 7: 3805 - 3817. PubMed
- Ibrahim ML, et al. 2018. Cell Rep. 25:3036. PubMed
- Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed
- Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
- Somogyi E, et al. 2021. Front Genet. 12:684152. PubMed
- Prakash MD, et al. 2021. PLoS One. 16:e0247492. PubMed
- Zhao J, et al. 2013. J Immunol Methods. 22:209. PubMed
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- Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
- Qin J, et al. 2020. Endocrinology. 161(4):bqaa033. PubMed
- RRID
- AB_315363 (BioLegend Cat. No. 505009) AB_315364 (BioLegend Cat. No. 505010)
- Structure
- Acid-labile cytokine, dimer, 17-21 kD (Mammalian)
- Cell Sources
- Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
- Cell Targets
- T cells, B cells, mast cells, macrophages
- Receptors
- IL-10R (CDw210)
- Cell Type
- Tregs
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefy R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today 13:198.
4. Quesniaux V. 1992. Res. Immunol. 143:385.
5. Norton SK, et al. 2008. J. Immunol. 180:2848.
- Regulation
- Downregulated by IL-4, IL-10
- Gene ID
- 16153 View all products for this Gene ID
- UniProt
- View information about IL-10 on UniProt.org
