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Alexa Fluor® 488 anti-mouse IL-10 Antibody
产品名称：
Alexa Fluor® 488 anti-mouse IL-10 Antibody
产品类别：
抗体
产品编号：
505013
产品应用：
505013
[价格]
规格 价格 库存
100µg ￥ 3180 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IL-10
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Application Notes

ELISA or ELISPOT Detection1,9,11: The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 & 504904) as the capture antibody.
ELISA Capture: The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. No. 505003) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard.
Neutralization14: The Ultra-LEAF? purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Flow Cytometry3: The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations.
Additional reported applications (for relevant formats) include: immunohistochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Simkin G, et al. 2000. J. Immunol. 164:2457.
  2. Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
  3. Khanna A, et al. 2000. J. Immunol. 164:1346.
  4. Sander B, et al. 1993. J. Immunol. Methods 166:201.
  5. Litton M, et al. 1994. J. Immunol. Methods 175:47.
  6. Andersson U, et al. 1999. Detection and qunatification of gene expression. New York:Springer-Verlag.
  7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
  8. Wang W, et al. 2004. FASEB J. 18:1043.
  9. Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
  10. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  11. Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
  12. Brummel R, et al. 2005. J. Immunol.174:2429. PubMed
  13. Kang YJ, et al. 2007. Stem Cells 25:1814. PubMed
  14. Seo N, et al. 2001. Immunology. 103:449. (Neut)
Product Citations
  1. Wang X, et al. 2021. Front Cell Dev Biol. 632805:9. PubMed
  2. Alves C, et al. 2015. PLoS One. 10: 0142972. PubMed
RRID
AB_493513 (BioLegend Cat. No. 505013)

Antigen Details

Structure
Acid-labile cytokine, dimer, 17-21 kD (Mammalian)
Cell Sources
Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
Cell Targets
T cells, B cells, mast cells, macrophages
Receptors
IL-10R (CDw210)
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefy R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today 13:198.
4. Quesniaux V. 1992. Res. Immunol. 143:385.
5. Norton SK, et al. 2008. J. Immunol. 180:2848.

Regulation
Downregulated by IL-4, IL-10
Gene ID
16153 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org

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