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Purified anti-human IL-10 Antibody
产品名称：
Purified anti-human IL-10 Antibody
产品类别：
抗体
产品编号：
501401
产品应用：
501401
[价格]
规格 价格 库存
50µg ￥ 996 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Rhesus, Cynomolgus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS - expressed, recombinant human IL-10
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IHC-P, Neut - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2.0 - 6.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture1-5 or ELISPOT Capture6: The Ultra-LEAFpurified JES3-9D7 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. No. 501502) as the detecting antibody and recombinant human IL-10 (Cat. No. 571009) as the standard. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture.
Neutralization1-3,9: The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 501427 & 501428). The JES3-9D7 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Additional reported applications (for the relevant formats) include:?immunohistochemical staining12.
Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max? Sets (Cat. Nos. 430601 & 430606) are specially developed and recommended.

The JES3-9D7 antibody reacts with human and viral interleukin-10 (IL-10).

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Capture, Neut)
  2. Gotlieb W, et al. 1992. Cytokine 4:385. (ELISA Capture, Neut)
  3. Yssel H, et al. 1992. J. Immunol. 149:2378. (ELISA Capture, Neut)
  4. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. (ELISA Capture)
  5. Burdin N, et al. 1993. J. Exp. Med. 177:295. (ELISA Capture)
  6. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Capture)
  7. Schaerli P, et al. 2000. J. Exp. Med. 192:1553.
  8. Jason J, et al. 1999. Clin. Diagn. Lab Immunol. 6:73.
  9. Akdis CA, et al. 1998. J. Clin. Invest. 102:98. (Neut)
  10. Stary G, et al. 2011. J. Immunol. 186:103. PubMed
  11. Mason GM, et al. 2012. PNAS. PubMed
  12. 12. Smith DR, et al. 1994. Am. J. Pathol. 145:18. (IHC)
Product Citations
  1. Chaturvedi V, et al. 2011. J Immunol. 186:6661. PubMed
  2. Ondigo BN, et al. 2018. Am J Trop Med Hyg. 98:1770. PubMed
  3. Ma F, et al. 2017. Exp Cell Res.. 10.1016/j.yexcr.2017.10.011. PubMed
  4. Subrahmanyam P and Maecker H. 2017. Curr Protoc Cytom.. 82:9.54.1-9.54.12.. PubMed
  5. Kurtaj A, et al. 2017. J Allergy Clin Immunol. 140:1746. PubMed
  6. Tai YT, et al. 2019. Leukemia. 33:426. PubMed
  7. Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed
  8. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  9. Schwabenland M, et al. 2021. Immunity. . PubMed
  10. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  11. Grech D, et al. 2016. J Acupunct Meridian Stud. 9:234-241. PubMed
  12. Mason G, et al. 2012. Proc Natl Acad Sci U S A. 109:14538. PubMed
  13. Stary G, et al. 2011. J Immunol. 186:103. PubMed
  14. Mou W, et al. 2017. BMC Immunol. 18:36. PubMed
RRID
AB_315167 (BioLegend Cat. No. 501401) AB_315168 (BioLegend Cat. No. 501402)

Antigen Details

Structure
Acid-labile cytokine, dimer, 35-40 kD (Mammalian)
Bioactivity
Inhibit IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppress delayed type hypersensitivity response; stimulate TH2 cell response; mast cell proliferation in
Cell Sources
Activated CD8+ and CD4+ T cells, activated monocytes, mast cells, Ly-1 B (mouse)
Cell Targets
T cells, B cells, mast cells, macrophages
Receptors
IL-10R (CDw210)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefyt R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today. 13:198.
4. Quesniaux V. 1992. Research Immunol. 143:385.

Regulation
Production inhibited by IL-4, IL-10
Gene ID
3586 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org

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