Ultra-LEAF™ Purified anti-human IL-10 Antibo-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2844	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-expressed recombinant human IL-10

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
IHC-F, ICC, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2.0 - 6.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture^1-4: The Purified JES3-19F1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. Nos. 501502 & 501503) as the detecting antibody. The Ultra-LEAF? Purified antibody is suggested for ELISPOT capture. For use as an ELISPOT capture antibody, a concentration range of 4.0 - 8.0 ?g/ml is recommended.
Flow Cytometry: The fluorochrome-labeled JES3-19F1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization^1,2: The Ultra-LEAF? Purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 506814 & 506815).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining^5,6 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 430601 to 430606) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Abrams J, et al. 1992. Immunol. Rev. 127:5.
Gotlieb W, et al. 1992. Cytokine 4:385.
Yssel H, et al. 1992. J. Immunol. 149:2378.
Burdin N, et al. 1993. J. Exp. Med. 177:295.
Andersson U, et al. 1999. Detection and quantification of gene expression. New York: Springer-Verlag.
Andersson J, et al. 1994. Immunology 83:16.

Product Citations

Gu C, et al. 2021. Front Immunol. 12:678036. PubMed

RRID

AB_2814433 (BioLegend Cat. No. 506813) AB_2814434 (BioLegend Cat. No. 506814)

Antigen Details

Structure: Acid-labile cytokine; dimer; 35-40 kD (Mammalian)
Bioactivity: Inhibit IFN-γ, TNF-β, IL-2 production by T_H1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppress delayed type hypersensitivity response; stimulate T_H2 cell response; mast cell proliferation in
Cell Sources: Activated CD8⁺ and CD4⁺ T cells, activated monocytes, mast cells, Ly-1 B (mouse)
Cell Targets: T cells, B cells, mast cells, macrophages
Receptors: IL-10R (CDw210)
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefyt R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today. 13:198.
4. Quesniaux V. 1992. Research Immunol. 143:385.
Regulation: Production inhibited by IL-4, IL-10
Gene ID: 3586 View all products for this Gene ID
UniProt: View information about IL-10 on UniProt.org

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