Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2.0 - 6.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

ELISA or ELISPOT Capture^1-4: The Purified JES3-19F1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. Nos. 501502 & 501503) as the detecting antibody. The Ultra-LEAF? Purified antibody is suggested for ELISPOT capture. For use as an ELISPOT capture antibody, a concentration range of 4.0 - 8.0 ?g/ml is recommended.
Flow Cytometry: The fluorochrome-labeled JES3-19F1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization^1,2: The Ultra-LEAF? Purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 506814 & 506815).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining^5,6 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 430601 to 430606) are specially developed and recommended.

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