Biotin anti-human IL-10 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1212	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS - expressed, recombinant human IL-10

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 500 to 2 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 ?g/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection^1-4 or ELISPOT Detection⁵: The biotinylated JES3-12G8 antibody is useful as a detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Ultra-LEAF? purified JES3-9D7 antibody (Cat. Nos. 501427 & 501428) or purified JES3-19F1 antibody (Cat. No. 506802/506810) as capture antibody and recombinant human IL-10 (Cat. No. 571009) as the standard.
Additional reported applications (for the relevant formats) include: immunohistochemical staining^6,7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, neutralization, and immunocytochemistry.
Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max? Sets (Cat. Nos. 430601 & 430606) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Abrams, J., et al. 1992. Immunol. Rev. 127:5.
Gotlieb, W., et al. 1992. Cytokine 4:385. (ELISA)
Yssel, H., et al. 1992 J. Immunol. 149:2378. (ELISA)
Burdin, N., et al. 1993. J. Exp. Med. 177:295. (ELISA)
Klinman, D., et al. 1994. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.19.
Tazi, A., et al. 1999. J. Immunol.163:3511
Andersson, J., et al. 1994. Immunology 83:16. (IHC)

Product Citations

Haug V, et al. 2020. Transplantation. e260:104. PubMed
Grech D, et al. 2016. J Acupunct Meridian Stud. 9:234-241. PubMed
Chan M, et al. 2021. Mol Syst Biol. 17:e10426. PubMed
Gilboa T, et al. 2020. Adv Healthc Mater. e2001111:. PubMed

RRID

AB_315177 (BioLegend Cat. No. 501501) AB_315178 (BioLegend Cat. No. 501502)

Antigen Details

Structure: Acid-labile cytokine; dimer; 35-40 kD (Mammalian)
Bioactivity: Inhibit IFN-γ, TNF-β, IL-2 production by T_H1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppress delayed type hypersensitivity response; stimulate T_H2 cell response; mast cell proliferation in
Cell Sources: Activated CD8⁺ and CD4⁺ T cells, activated monocytes, mast cells, Ly-1 B (mouse)
Cell Targets: T cells, B cells, mast cells, macrophages
Receptors: IL-10R (CDw210)
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. de Waal-Malefyt, R., et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard, M., et al. 1992. Immunol. Today. 13:198.
4. Quesniaux, V. 1992. Research Immunol. 143:385.
Regulation: Production inhibited by IL-4, IL-10
Gene ID: 3586 View all products for this Gene ID
UniProt: View information about IL-10 on UniProt.org

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