PerCP/Cyanine5.5 anti-human IL-13 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 2472	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-expressed, recombinant human IL-13

Formulation

test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

?g sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis.
For test sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
For ?g sizes, the suggested use of this reagent for immunofluorescent staining is ≤0.25 ?g per million cells in 100 ?l volume.
It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture^1,2: The Ultra-LEAF? Purified JES10-5A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5020 antibody (Cat. No. 502001) as the detecting antibody. The Ultra-LEAF? Purified antibody is suggested for ELISPOT capture.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

McKenzie, A., et al. 1994. Curr. Prot. Immunol.. John Wiley & Sons. New York. Section 6.18.
Abrams, J. 1995. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.20.
Schaerli, P., et al. 2000. J. Exp. Med. 192:1553.
Andersson, J., et al. 1994. Immunology 83:16.
Skansen-Saphir, U., et al. 1994. Eur. J. Immunol. 24:916.
Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
Gelfand, E. W., et al. 2006. J. Aller. and Clinic. Immunol. 117:577.
Knorr, C., et al. 1999. Am. J. Pathol. 155:2019.

Product Citations

Gu C, et al. 2021. Front Immunol. 12:678036. PubMed
Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
Fuery A, et al. 2015. PLoS One. 10: 0133126. PubMed
Poulsen L 2012. J Immunol. 189:4431. PubMed

RRID

AB_2124283 (BioLegend Cat. No. 501912) AB_2124284 (BioLegend Cat. No. 501911)

Antigen Details

Structure: Cytokine; 17 kD (Mammalian)
Bioactivity: Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes
Cell Sources: Activated T cells, mast cells, NK cells
Cell Targets: B cells, monocytes, macrophages, basophils
Receptors: Moderate affinity IL-13Rα1; high affinity heterodimer IL-13Rα1/IL-4Rα also binds IL-4; both receptor complexes have γ-subunit in common with IL-2R, IL-4R, IL-7R, IL-15R ; high affinity IL-13Rα2 incapable of signaling
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hilton, D., et al. 1996. P. Natl. Acad. Sci. USA 93:497.
3. Obiri, N., et al. 1995. J. Biol. Chem. 270:8797.
4. Ochensberger, B., et al. 1996. Blood 88:3028.
Regulation: IL-13Rα2 possible antagonist
Gene ID: 3596 View all products for this Gene ID
UniProt: View information about IL-13 on UniProt.org

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