Brilliant Violet 421™ anti-human IL-13 Antib-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2112	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-expressed, recombinant human IL-13

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

ELISA or ELISPOT Capture^1,2: The Ultra-LEAF? Purified JES10-5A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5020 antibody (Cat. No. 502001) as the detecting antibody. The Ultra-LEAF? Purified antibody is suggested for ELISPOT capture.

Application References (PubMed link indicates BioLegend citation)

McKenzie, A., et al. 1994. Curr. Prot. Immunol.. John Wiley & Sons. New York. Section 6.18.
Abrams, J. 1995. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.20.
Schaerli, P., et al. 2000. J. Exp. Med. 192:1553.
Andersson, J., et al. 1994. Immunology 83:16.
Skansen-Saphir, U., et al. 1994. Eur. J. Immunol. 24:916.
Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
Gelfand, E. W., et al. 2006. J. Aller. and Clinic. Immunol. 117:577.
Knorr, C., et al. 1999. Am. J. Pathol. 155:2019.

Product Citations

Solforosi L, et al. 2021. J Exp Med. :218. PubMed
Punik J, et al. 2021. Cell Reports. 35(13):109320. PubMed
Wang SQ, et al. 2021. J Viral Hepat. 28:795. PubMed

RRID

AB_2616747 (BioLegend Cat. No. 501915) AB_2616748 (BioLegend Cat. No. 501916)

Antigen Details

Structure: Cytokine; 17 kD (Mammalian)
Bioactivity: Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes
Cell Sources: Activated T cells, mast cells, NK cells
Cell Targets: B cells, monocytes, macrophages, basophils
Receptors: Moderate affinity IL-13Rα1; high affinity heterodimer IL-13Rα1/IL-4Rα also binds IL-4; both receptor complexes have γ-subunit in common with IL-2R, IL-4R, IL-7R, IL-15R ; high affinity IL-13Rα2 incapable of signaling
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hilton, D., et al. 1996. P. Natl. Acad. Sci. USA 93:497.
3. Obiri, N., et al. 1995. J. Biol. Chem. 270:8797.
4. Ochensberger, B., et al. 1996. Blood 88:3028.
Regulation: IL-13Rα2 possible antagonist
Gene ID: 3596 View all products for this Gene ID
UniProt: View information about IL-13 on UniProt.org

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