Purified anti-human IL-5 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
500µg	￥ 3144	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-expressed, recombinant human IL-5

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture - Quality tested
IHC-F, ICC, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1.0 - 4.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-5 recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA^1-3 or ELISPOT^4-6 Capture: The purified JES1-39D10 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the biotinylated JES1-5A10 (Cat. No. 501002) antibody as the detecting antibody. The LEAF? purified antibody is suggested for ELISPOT capture.
Neutralization¹: The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500907).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining^8,9 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 430401 & 430404) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Abrams, J., et al. 1992. Immunol. Rev. 127:5.
Butterfield, J., et al. 1992. Blood 79:688.
Abrams, J., et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker, New York. p.133.
Klinman, D., et al. 1994. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.19.6.
Mahanty, S., et al. 1992. J. Immunol. 148:3567.
Limaye, A., et al. 1991. J. Clin. Inv. 88:1418.
Prussin, C., et al. 1995. J. Immunol. Meth. 188:117.
Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
Andersson, J., et al. 1994. Immunology 83:16.

Product Citations

Tripathi T, et al. 2019. Immunohorizons. 0.201388889. PubMed

RRID

AB_315137 (BioLegend Cat. No. 500902)

Antigen Details

Structure: Cytokine; dimer; 45 kD (Mammalian)
Bioactivity: Growth/differentiation of eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Cell Sources: Mast cells, T cells, bone marrow stromal cells
Cell Targets: Eosinophils
Receptors: Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immun. 35:593.
Regulation: Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID: 3567 View all products for this Gene ID
UniProt: View information about IL-5 on UniProt.org

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