PE anti-human IL-5 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1248	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-expressed, recombinant human IL-5

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

ELISA^1-3 or ELISPOT^4-6 Capture: The purified JES1-39D10 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the biotinylated JES1-5A10 (Cat. No. 501002) antibody as the detecting antibody. The LEAF? purified antibody is suggested for ELISPOT capture.
Neutralization¹: The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500907).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining^8,9 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 430401 & 430404) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Abrams, J., et al. 1992. Immunol. Rev. 127:5.
Butterfield, J., et al. 1992. Blood 79:688.
Abrams, J., et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker, New York. p.133.
Klinman, D., et al. 1994. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.19.6.
Mahanty, S., et al. 1992. J. Immunol. 148:3567.
Limaye, A., et al. 1991. J. Clin. Inv. 88:1418.
Prussin, C., et al. 1995. J. Immunol. Meth. 188:117.
Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
Andersson, J., et al. 1994. Immunology 83:16.

Product Citations

Golebski K, et al. 2019. Nat Commun. 10:2162. PubMed
Numazaki M, et al. 2021. J Pharmacol Exp Ther. :JPET-AR-2021-000686. PubMed
Dai G, et al. 2021. JCI Insight. 6:. PubMed
Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed

RRID

AB_315138 (BioLegend Cat. No. 500903) AB_315139 (BioLegend Cat. No. 500904)

Antigen Details

Structure: Cytokine; dimer; 45 kD (Mammalian)
Bioactivity: Growth/differentiation of eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Cell Sources: Mast cells, T cells, bone marrow stromal cells
Cell Targets: Eosinophils
Receptors: Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immun. 35:593.
Regulation: Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID: 3567 View all products for this Gene ID
UniProt: View information about IL-5 on UniProt.org

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