Biotin anti-human DR3 (TRAMP) Antibody
- 产品名称:
- Biotin anti-human DR3 (TRAMP) Antibody
- 产品类别:
- 抗体
- 产品编号:
- 307104
- 产品应用:
- 307104
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- human DR3-Ig fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: The JD3 antibody is useful for immunofluorescent staining and flow cytometric analysis of DR3/TRAMP receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307106) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 307104) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
- Application References
(PubMed link indicates BioLegend citation) -
- Nakayama M, et al. 2002. J. Immunol. 168:734.
- RRID
- AB_2207081 (BioLegend Cat. No. 307104)
- Structure
- TNFR superfamily
- Distribution
- Activated lymphocytes and thymocytes
- Function
- Lymphocyte homeostasis
- Ligand/Receptor
- Apo-3L, TWEAK
- Cell Type
- Lymphocytes, Thymocytes
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
-
1. Nakayama M, et al. 2002. J. Immunol. 168:734.
2. Nakayama M, et al. 2000. J. Exp. Med. 192:1373.
- Gene ID
- 8718 View all products for this Gene ID
- UniProt
- View information about DR3 on UniProt.org