APC anti-human/mouse CD266 (Fn14, TWEAK R) Antibod
- 产品名称：
- APC anti-human/mouse CD266 (Fn14, TWEAK R) Antibod
- 产品类别：
- 抗体
- 产品编号：
- 314107
- 产品应用：
- 314107
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Fn14 transfected P815
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting3,4, immunofluorescence3,4, and immunohistochemistry of frozen sections5 and formalin-fixed paraffin-embedded sections3. ITEM-4 antibody has no effect on TWEAK-induced MAPK activation in RAW cells1.
- Application References
(PubMed link indicates BioLegend citation) -
- Polek TC, et al. 2003. J. Biol. Chem. 278:32317.
- Nakayama M, et al. 2003. J. Immunol. 170:341.
- Karaca G, et al. 2014. PLoS One. 9:83987. (IHC, IF, WB) PubMed
- Mu?oz-García B, et al. 2006. Stroke. 37:2044. (IF, WB)
- Jakubowski A, et al. 2005. J. Clin. Invest. 115:2955. (IHC)
- Karaca G, et al. 2015. Am J Physiol Gastrointest Liver Physiol. 308:325. PubMed
- Product Citations
-
- Deng CC, et al. 2021. Nat Commun. 12:3709. PubMed
- RRID
- AB_2800830 (BioLegend Cat. No. 314107) AB_2810477 (BioLegend Cat. No. 314108)
- Structure
- Transmembrane TNF superfamily receptor, contains no death domain, 14 kD
- Distribution
-
Widely expressed in heart, placenta, lung, muscle, kidney, pancreas, and HUVEC
- Function
- Binds to TWEAK (CD255), thought to play a role in cell death and inflammation
- Ligand/Receptor
- TWEAK (CD255); interacts with cytoplasmic proteins TRAF1, 2, and 3
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Feng SL, et al. 2000. Am. J. Path. 156:1253.
2. Meighan-Mantha RL, et al. 1999. J. Biol. Chem. 274:33166.
3. Nakayama M, et al. 2003. J. Immunol. 170:341.
4. Lynch CN, et al. 1999. J. Biol. Chem. 274:8455.
- Gene ID
- 27279 View all products for this Gene ID 51330 View all products for this Gene ID
- UniProt
- View information about CD266 on UniProt.org
