Purified anti-human CD86 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 924	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Capuchin Monkey, Common Marmoset, Cotton-topped Tamarin, Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
IHC-F, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per 10⁶ cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁶, Western blotting³, and blocking of T cell activation^2,4,5. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 305449 & 305450).

Application References (PubMed link indicates BioLegend citation)

Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Dieu M. 1998. J. Exp. Med. 188:373. (Block)
Esser M, et al. 2001. J. Virol. 75:6173. (WB)
Jeannin P, et al. 1999. J. Immunol. 162:2044. (Block)
Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107. (Block)
Geissmann F, et al. 2001. Blood 97:1241. (IHC)

Product Citations

Melo-Gonzalez F, et al. 2018. J Biol Chem. 293:8543. PubMed
Mask E, et al. 2022. Front Cell Infect Microbiol. 12:880860. PubMed
Laudanski K, et al. 2018. J Transl Med. 0.765972222. PubMed
Nolan E, et al. 2017. Sci Transl Med. 9:eaal4925. PubMed
Raffray L, et al. 2020. Immunology. 159:88. PubMed
Azari S, et al. 2021. MBio. 12:e0184921. PubMed
Syrimi E, et al. 2021. iScience. 24:103215. PubMed
Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
Gruber CN, et al. 2020. Cell. 183:982. PubMed
Soskic B, et al. 2021. Front Immunol. 11:577655. PubMed
Jog NR, et al. 2018. Front Immunol. 9:2198. PubMed
Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
Speir M,et al. 2017. Sci Rep.. 10.1038/s41598-017-14690-5. PubMed
Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
Ehinger E, et al. 2021. Cardiovasc Res. 117:1166. PubMed
Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
Jayakumar A, et al. 2008. Infect Immun. 76:2138. PubMed
Zimmerman MG, et al. 2018. Cell Host Microbe. 24:731. PubMed
Eriksson E, et al. 2017. Clin Cancer Res. 5.018055556. PubMed
Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed

RRID

AB_314521 (BioLegend Cat. No. 305401) AB_314522 (BioLegend Cat. No. 305402)

Antigen Details

Structure: Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
Distribution: Monocytes/macrophages, activated B cells and T cells, dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28, CD152
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Hathcock K, et al. 1996. Adv. Immunol. 62:131.
2. June C, et al. 1994. Immunol. Today 15:321.
Gene ID: 942 View all products for this Gene ID
UniProt: View information about CD86 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们