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Pacific Blue™ anti-human CD86 Antibody
产品名称：
Pacific Blue™ anti-human CD86 Antibody
产品类别：
抗体
产品编号：
305417
产品应用：
305417
[价格]
规格 价格 库存
25µg ￥ 1488 1

产品详情

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Capuchin Monkey, Common Marmoset, Cotton-topped Tamarin, Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
?g sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
test size: lot-specific; ?g sizes: 0.5 mg/ml
Storage & Handling
The CD86 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 ?l per 106 cells in 100 ?l volume.
For ?g sizes, the suggested use of this reagent for immunofluorescent staining is ≤0.5 ?g per 106 cells in 100 ?l volume.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, Western blotting3, and blocking of T cell activation2,4,5. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 305449 & 305450).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Dieu M. 1998. J. Exp. Med. 188:373. (Block)
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Jeannin P, et al. 1999. J. Immunol. 162:2044. (Block)
  5. Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107. (Block)
  6. Geissmann F, et al. 2001. Blood 97:1241. (IHC)
Product Citations
  1. Feuerer N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  2. Groen R, et al. 2015. J Leukoc Biol. 98: 623 - 630. PubMed
  3. Gustafsson R, et al. 2013. PLoS One. 8:e58122. PubMed
  4. Magalh?es L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  5. Thwe PM et al. 2017. Cell metabolism. 26(3):558-567 . PubMed
  6. Beljanski V, et al. 2015. J Virol. 89: 10612 - 10624. PubMed
  7. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  8. Mansour H, et al. 2010. J Infect Dis. 202:1671. PubMed
  9. Séguier S, et al. 2013. PLoS One. 8:70937. PubMed
  10. Soema P, et al. 2015. Eur J Pharm Biopharm. 94: 427-435. PubMed
  11. Tavano B, et al. 2013. J Immunol. 190:2622. PubMed
  12. Chao Y, et al. 2014. J Leukoc Biol. 96:1037. PubMed
  13. Liu W, et al. 2012. Vaccine. 30:1659. PubMed
  14. Teirlinck A, et al. 2015. Infect Immun . 83: 3732-3739. PubMed
  15. Brummelman J, et al. 2015. Infect Immun . 83:227. PubMed
  16. Tan EE, et al. 2020. J Clin Invest. 130:5817. PubMed
  17. Rusconi M, et al. 2017. Sci Rep. 5.745138889. PubMed
RRID
AB_493662 (BioLegend Cat. No. 305417) AB_493663 (BioLegend Cat. No. 305418) AB_2260067 (BioLegend Cat. No. 305423)

Antigen Details

Structure
Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
Distribution

Monocytes/macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28, CD152
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Hathcock K, et al. 1996. Adv. Immunol. 62:131.
2. June C, et al. 1994. Immunol. Today 15:321.

Gene ID
942 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org

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