Brilliant Violet 421™ anti-human CD86 Antibo-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2448	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Capuchin Monkey, Common Marmoset, Cotton-topped Tamarin, Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁶, Western blotting³, and blocking of T cell activation^2,4,5. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 305449 & 305450).

Application References (PubMed link indicates BioLegend citation)

Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Dieu M. 1998. J. Exp. Med. 188:373. (Block)
Esser M, et al. 2001. J. Virol. 75:6173. (WB)
Jeannin P, et al. 1999. J. Immunol. 162:2044. (Block)
Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107. (Block)
Geissmann F, et al. 2001. Blood 97:1241. (IHC)

Product Citations

Wenthe J, et al. 2021. Cancer Immunology Immunotherapy. . PubMed
Eriksson E, et al. 2016. Gene Ther. 10.1038/gt.2016.80. PubMed
Woolsey C et al. 2019. Cell Rep. 28(12):3032-3046 . PubMed
Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
Arenas EJ, et al. 2021. Nat Commun. 12:1237. PubMed
Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
Upasani V, et al. 2019. Front Immunol. 2.152777778. PubMed

RRID

AB_10899582 (BioLegend Cat. No. 305425) AB_11204252 (BioLegend Cat. No. 305426)

Antigen Details

Structure: Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
Distribution: Monocytes/macrophages, activated B cells and T cells, dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28, CD152
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Hathcock K, et al. 1996. Adv. Immunol. 62:131.
2. June C, et al. 1994. Immunol. Today 15:321.
Gene ID: 942 View all products for this Gene ID
UniProt: View information about CD86 on UniProt.org

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