Biotin anti-human CD86 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2820	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Capuchin Monkey, Common Marmoset, Cotton-topped Tamarin, Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁶, Western blotting³, and blocking of T cell activation^2,4,5. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 305449 & 305450).

Application References (PubMed link indicates BioLegend citation)

Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Dieu M. 1998. J. Exp. Med. 188:373. (Block)
Esser M, et al. 2001. J. Virol. 75:6173. (WB)
Jeannin P, et al. 1999. J. Immunol. 162:2044. (Block)
Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107. (Block)
Geissmann F, et al. 2001. Blood 97:1241. (IHC)

Product Citations

Lee A, et al. 2011. Mol Immunol. 48:1160. PubMed
Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed

RRID

AB_314524 (BioLegend Cat. No. 305404)

Antigen Details

Structure: Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
Distribution: Monocytes/macrophages, activated B cells and T cells, dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28, CD152
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Hathcock K, et al. 1996. Adv. Immunol. 62:131.
2. June C, et al. 1994. Immunol. Today 15:321.
Gene ID: 942 View all products for this Gene ID
UniProt: View information about CD86 on UniProt.org

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