Pacific Blue™ anti-IRF4 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1692	1

Product Details

Verified Reactivity

Mouse, Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

GST fusion protein containing C-terminal of murine IRF4

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Pacific Blue? under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.25??g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.?

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: spatial biology (IBEX)^3,4.

Application References (PubMed link indicates BioLegend citation)

Zheng Y, et al. 2009. Nature 458:351
Yin SY, et al. 2011. Exp Cell Res. 317:2210. PubMed
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

RRID

AB_2814496 (BioLegend Cat. No. 646417) AB_2814497 (BioLegend Cat. No. 646418)

Antigen Details

Structure: Molecular weight is approximately 51 kD.
Distribution: IRF4 is reported to be expressed exclusively in the immune system.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Lu R. 2008. Trends Immunol 29:487.
Gene ID: 3662 View all products for this Gene ID
UniProt: View information about IRF4 on UniProt.org

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