Alexa Fluor® 647 anti-IRF4 Antibody
- 产品名称：
- Alexa Fluor® 647 anti-IRF4 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 646407
- 产品应用：
- 646407
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- GST fusion protein containing C-terminal of murine IRF4
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis.?For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.
- Application References
(PubMed link indicates BioLegend citation) -
- Zheng Y, et al. 2009. Nature 458:351
- Yin SY, et al. 2011. Exp Cell Res. 317:2210. PubMed
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Wang D, et al. 2018. Immunity. 48:659. PubMed
- Mondala PK, et al. 2021. Cell Stem Cell. 28(4):623-636.e9. PubMed
- Qian J, et al. 2021. Int J Mol Sci. 22:. PubMed
- Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
- Kiuchi M, et al. 2021. J Exp Med. 218:. PubMed
- Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed
- Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed
- Chen PM, et al. 2022. Sci Adv. 8:eabo4271. PubMed
- Vora AA, et al. 2021. STAR Protocols. 2(2):100565. PubMed
- RRID
- AB_2564047 (BioLegend Cat. No. 646407) AB_2564048 (BioLegend Cat. No. 646408)
