Alexa Fluor® 488 anti-IRF4 Antibody
- 产品名称：
- Alexa Fluor® 488 anti-IRF4 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 646405
- 产品应用：
- 646405
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- GST fusion protein containing C-terminal of murine IRF4
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis.?For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.
- Application References
(PubMed link indicates BioLegend citation) -
- Zheng Y, et al. 2009. Nature 458:351
- Yin SY, et al. 2011. Exp Cell Res. 317:2210. PubMed
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Ford K, et al. 2020. Cancer Res. 80:1846. PubMed
- Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
- Moon HG et al. 2018. Immunity. 49(2):275-287 . PubMed
- Zhou WJ, et al. 2022. Sci Adv. 8:eabj2488. PubMed
- Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
- Utz SG, et al. 2020. Cell. 181(3):557-573. PubMed
- Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
- Jenks SA et al. 2018. Immunity. 49(4):725-739 . PubMed
- RRID
- AB_2563266 (BioLegend Cat. No. 646405) AB_2563267 (BioLegend Cat. No. 646406)
