Pacific Blue™ anti-human TCR α/β
- 产品名称:
- Pacific Blue™ anti-human TCR α/β
- 产品类别:
- 抗体
- 产品编号:
- 306715
- 产品应用:
- 306715
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone. ?Other anti-CD3 clones may compete out the binding of IP26. ?
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. (FC)
- Joseph A, et al. 2008. J. Virol. 82:3078. (FC) PubMed
- Pinto JP, et al. 2010. Immunology. 130:217. PubMed
- Product Citations
-
- Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
- Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
- Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
- Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
- RRID
- AB_1953256 (BioLegend Cat. No. 306715) AB_1953257 (BioLegend Cat. No. 306716)